A microscopy trough is a special kind of Langmuir trough for characterization of Langmuir films before, during and after fabrication.
A microscopy trough is a special kind of Langmuir trough that includes a sapphire window in the top base of the PTFE trough that allows high optical transmission down to a wavelength of 200 nm (suitable for visible light or UV microscopy). It enables characterization of Langmuir films before, during and after fabrication. Troughs suitable for both upright and inverted microscopes are available.
UV-visible absorption spectroscopy of monolayers on the Langmuir Trough can be used to determine how light-absorbing molecules interact with one another when a monolayer of the molecules, or mixtures of molecules, are compressed to various surface pressures. The molecular neighbor-neighbor separation and packing density is made possible by controlling the surface pressure of the monolayer.
When photoluminescent materials are studied, the Inverted Microscopy Trough allows a microscope objective lens to reach within 2.2 mm of the monolayer, from beneath, to enable stimulation of the monolayer with very low wavelength light from above. The transmission properties of the sapphire microscopy window and the glass inverted microscopy window are shown below.