Immobilization of sensor molecules into Langmuir-Blodgett and Langmuir-Schaefer deposited layers can be used in variety of electrical, optical and biomedical applications.

Sensors can be fabricated from biochemical molecules where proteins, DNA or sugars are used as detector molecules and are incorporated into a phospholipid monolayer. Other candidate molecules include organic or inorganic dyes, nanoparticles or other functional molecules that give a desired response. These can be incorporated into a coating with Langmuir-Blodgett, Langmuir-Schaefer or dip coating to give a thin layer on an electrode or other probe.

Immobilized proteins used as sensors

Immobilization of Alcohol Dehydrogenase in Phospholipid Langmuir-Blodgett Films To Detect Ethanol

Luciano Caseli, Angelo C. Perinotto, Tapani Viitala, Valtencir Zucolotto, and Osvaldo N. Oliveira, Jr.

Langmuir (2009), 25, 3057-3061

Alcohol dehydrogenase (ADH) mixed in dimyristoylphosphatidic acid (DMPA) monolayer exhibited enhanced transfer and detection ability towards ethanol compared to pure ADH layers. Studies proved that the ADH structure remained unchanged over one month in the mixed monolayer under storage conditions. A sensor array deposited on gold electrodes could detect alcohol down to 10 ppb concentration.

For more details of the study please check the original publication.

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