No instrument-to-instrument variation on Sophion Qube384
Posted on May 30, 2016
In electrophysiology literature values for IC50 can range from a few nM to several hundred µM for a specific compound. Variations are caused by many parameters such as the cell line itself, the growth conditions, measurement platform, voltage protocol, solutions etc.
To access the repeatability (inter-instrument variation) of Sophion Qube384 we performed a few studies on hERG with well-known reference compounds (Cisapride, Terfenadine and Verapamil). Studies were performed on two different Qube instruments with same protocols on the same day.
We found that inter-instrument variation was very low with IC50 values with a max/min ratio of 1.08, 1.01 and 1.38 respectively for the three reference compounds.