An electronically enhanced chiral sum frequency generation vibrational spectroscopy study of lipid-bound cytochrome c

Electronically enhanced chiral SFG spectroscopy was employed to study the lipid bound cyt c in situ. It was directly observed that upon interacting with anionic phospholipids, the amino acid residues around the heme adopted the beta-sheet conformation. In addition, the orientation of this newly formed beta-sheet structure was found to be sensitive to the bulk pH.