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    Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine

    Year: 2018

    Journal: J. Inorg. Biochem., Volume 178, JAN, page 43–53

    Authors: Forte, Lucia; Torricelli, Paola; Bonvicini, Francesca; Boanini, Elisa; Gentilomi, Giovanna Angela; Lusvardi, Gigliola; Della Bella, Elena; Fini, Milena; Nepita, Edoardo Vecchio; Bigi, Adriana

    Organizations: Rizzoli Orthopaedic Institute [6562]; University of Bologna [RFO 2015/16]

    Keywords: Silver nanoparticles; Octacalcium phosphate; alpha-Tricalcium phosphate; Polydopamine; Multi-drug resistant bacteria; Osteoblast

    In this work we developed new antibacterial composite materials using polydopamine (PDA) to trigger the deposition of silver nanoparticles (AgNPs) onto calcium phosphates, namely octacalcium phosphate (OCP) and a-tricalcium phosphate (alpha TCP). Functionalization of OCP and alpha TCP with a self-polymerized polydopamine layer was obtained by soaking the calcium phosphates in dopamine solution. The PDA surface of functionalized calcium phosphates (OCPd and alpha TCPd) promoted the deposition of AgNPs by reducing silver ions when soaked in a silver nitrate solution. The amount of deposited AgNPs can be modulated by varying the concentration of silver nitrate solution and the type of substrate. The results of in vitro tests carried out with osteoblast-like MG63 cells indicate that the combination of AgNPs with OCP provides more biocompatible materials than those obtained using alpha TCP as substrate. In particular, the study of osteoblast activity and differentiation was focused on the samples OCPdAg5 (silver content = 8.2 wt%) and alpha TCPdAg5 (silver content = 4.7 wt%), which did not show any cytotoxicity, and compared with those obtained on pure OCP and aTCP. The results demonstrate that the AgNPs loaded materials support osteoblast viability and differentiation, whereas they significantly inhibit the growth of relevant antibiotic-resistant pathogenic bacteria.
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