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    CMC-Modified Cellulose Biointerface for Antibody Conjugation

    Year: 2012

    Journal: Biomacromolecules, 2012, 13 (4), pp 1051–1058, 20120618

    Authors: Hannes Orelma*†, Tuija Teerinen‡, Leena-Sisko Johansson†, Susanna Holappa†, and Janne Laine†

    Last authors: Janne Laine

    Organizations: † Aalto University, School of Chemical Technology, Department of Forest Products Technology, Espoo, Finland ‡ VTT Technical Research Centre of Finland, 02044 VTT, Espoo, Finland

    Country: Finland

    In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir–Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.