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    Collagen-immobilized polyimide membranes for retinal pigment epithelial cell adherence and proliferation

    Year: 2017

    Journal: Cogent Chem., Volume 3

    Authors: Teymouri, Shokoufeh; Calejo, Maria Teresa; Hiltunen, Maiju; Sorkio, Anni; Juuti-Uusitalo, Kati; Skottman, Heli; Kellomaki, Minna

    Organizations: Health Research Council of the Academy of Finland [218050, 137801]; Academy of Finland [253134]; Japan Science and Technology Agency of the Academy of Finland [253134]; program of Human Spare Parts of TEKES-The Finnish Funding Agency for Innovation

    Keywords: polyimide; chemical covalent modification; acrylic acid grafting; collagen IV immobilization; retinal pigment epithelial cells

    Degenerative retinal diseases are a leading cause of visual loss and irreversible blindness, particularly in the developed world. Retinal pigment cell (RPE) transplantation is nowadays considered the most promising therapeutic approach for certain retinal diseases, and the presence of a supportive scaffold has been considered essential to ensure the success of the implant. In this work, collagen IV was covalently immobilized to the surface of polyimide membranes, with the purpose of developing scaffold materials for RPE cell culture. The covalent modification method involved four steps: argon-plasma treatment, acrylic acid graft polymerization, surface activation, and finally immobilization of collagen type IV. Collagen-modified membranes did not become more rough but became significantly more hydrophilic than the unmodified and dip-coated controls. ARPE-19 cell morphology and attachment were studied by immunofluorescence staining and confocal microscopy. Covalently modified surfaces showed cell attachment and cell properties comparable to the uncoated and dip-coated controls. This work demonstrated the potential of collagen IV-immobilized polyimide membranes as substrates for the growth of ARPE-19 cells.
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