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    Control of Neural Stem Cell Adhesion and Density by an Electronic Polymer Surface Switch

    Year: 2008

    Journal: Langmuir, 2008, 24 (24), pp 14133–14138, 20111221

    Authors: Carmen Salt, Emilien Saindon, Maria Bolin, Anna Kanciurzewska, Mats Fahlman, Edwin W. H. Jager, Pentti Tengvall, Ernest Arenas and Magnus Berggren

    Organizations: Molecular Neurobiology, MBB, Karolinska Institutet, SE-171 77 Stockholm, Sweden, Organic Electronics, ITN, Linkping University, SE-601 74 Norrkping, Sweden, Organic Physics, ITN, Linkping University, SE-601 74 Norrkping, Sweden, and Applied Physics, IFM, Linkping University, SE-581 83 Linkping, Sweden

    Adhesion is an essential parameter for stem cells. It regulates the overall cell density along the carrying surface, which further dictates the differentiation scheme of stem cells toward a more matured and specified population as well as tissue. Electronic control of the seeding density of neural stem cells (c17.2) is here reported. Thin electrode films of poly(3,4-ethylenedioxythiophene) (PEDOT):Tosylate were manufactured along the floor of cell growth dishes. As the oxidation state of the conjugated polymer electrodes was controlled, the seeding density could be varied by a factor of 2. Along the oxidized PEDOT:Tosylate-electrodes, a relatively lower density of, and less tightly bonded, human serum albumin (HSA) was observed as compared to reduced electrodes. We found that this favors adhesion of the specific stem cells studied. Surface analysis experiments, such as photoelectron spectroscopy, and water contact angle measurements, were carried out to investigate the mechanisms responsible for the electronic control of the seeding density of the c17.2 neural stem cells. Further, our findings may provide an opening for electronic control of stem cell differentiation.