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    Controlled Density Patterning of Tolylterpyridine-Tagged Oligonucleotides

    Year: 2011

    Journal: Langmuir, 2011, 27 (14), pp 8595–8599, 20110906

    Authors: Tuccitto N. †, Giamblanco N. †, Ghosh S. ‡, Spampinato V. †, Labbe P. ‡, Dumy P. ‡, Quici S.§, Marletta G.†, Defrancq E. *‡, Licciardello A. *†

    Last authors: Antonino Licciardello

    Organizations: Laboratory for Molecular Surfaces and Nanotechnology (LAMSUN), Dipartimento di Scienze Chimiche, Universita degli Studi di Catania and CSGI, Via A. Doria 6, 95125 Catania, Italy Departement de Chimie Molculaire UMR CNRS 5250, Universite Joseph Fourier, BP 53, 38041 Grenoble cedex 9, France Istituto di Scienze e Tecnologie Molecolari del CNR, 20133 Milano, Italy

    Country: France, Italy

    An efficient surface anchoring strategy of tolylterpyridine-tagged DNA single strands (ssDNA-ttpy) synthesized on gold electrodes is reported. The method is based on exchange reactions between Fe(II)bis-terpyridine complexed SAMs and ssDNA-ttpy, and allows efficient hybrydization of the cDNA strands. Moreover, by using low-current focused ion beam lithography, micropatterned arrays are obtained, homogeneously covered with ssDNA-ttpy. The surface adsorption kinetics of ssDNA-ttpy, as well as its hybridization efficiency, was monitored by in situ quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The effective confinement of the ssDNA-ttpy at the micrometer level has been monitored by time of flight secondary ion mass spectrometry (ToF-SIMS) and ellipsometric surface imaging experiments, providing laterally resolved chemical and topographic mapping.