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    Designing of Collagen Based Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Scaffolds for Tissue Engineering

    Year: 2015

    Journal: INTERNATIONAL JOURNAL OF POLYMER SCIENCE, Vol. , p -, 20170208

    Authors: Vigneswari, S.; Khalil, H. P. S. Abdul; Amirul, A. A.

    Organizations: MOSTI, NIBM, Malaysian Inst Pharmaceut & Nutraceut, George Town 11700, Malaysia; Univ Malaysia Terengganu, Inst Marine Biotechnol, Kuala Terengganu 21030, Malaysia; Univ Sains Malaysia, Sch Ind Technol, George Town 11800, Malaysia; Univ Sains Malaysia, Sch Biol Sci, George Town 11800, Malaysia; Univ Sains Malaysia, Ctr Chem Biol, George Town 11900, Malaysia

    P(3HB-co-4HB) copolymer was modified using collagen by adapting dual solvent system. The surface properties of samples were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), organic elemental analysis (CHN analysis), and water contact angle measurements. The effects of collagen concentration, scaffold thickness, and 4HB molar fraction on the hydrophilicity were optimized by the Taguchi method. The orthogonal array experiment was conducted to obtain the response for a hydrophilic scaffold. Analysis of variance (ANOVA) was used to determine the significant parameters and determine the optimal level for each parameter. The results also showed that the hydrophilicity of P(3HB-co-4HB)/collagen blend scaffolds increased as the collagen concentration increased up to 15 wt% with a molar fraction of 50 mol% at 0.1 mm scaffold thickness. The biocompatibility of the P(3HB-co-4HB)/collagen blend surface was evaluated by fibroblast cell (L929) culture. The collagen blend scaffold surfaces showed significant cell adhesion and growth as compared to P(3HB-co-4HB) copolymer scaffolds.