Contact
    Start Publications Endothelial Cell Culture Under Perfusion On A Polyester-Toner ...
    ←  Publication Finder
    Attension

    Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device

    Year: 2017

    Journal: Sci Rep, Volume 7, SEP 5

    Authors: Urbaczek, Ana Carolina; Gomes Carneiro Leao, Paulo Augusto; Ribeiro de Souza, Fayene Zeferino; Afonso, Ana; Alberice, Juliana Vieira; Dias Cappelini, Luciana Teresa; Carlos, Iracilda Zeppone; Carrilho, Emanuel

    Organizations: CNPq [150250/2014-2, 150096/2014-3, 400168/2013-8, 311323/2011-1, 482696/2011-7]; Laboratorio de Eletronica Organica "Prof. Guilherme Fontes Ferreira Leal" - IFSC-USP

    This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our "vein-on-a-chip" mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.
    View publication