Enhancement of neuronal cell adhesion by covalent binding of poly-d-lysine

We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalentbinding between the poly-d-Lysine and substrates and investigated neuronalcelladhesion properties and cell growth morphology. The number of neuronalcell and the number of neurite per neuronalcell on PDL bound surfaces was much more than those on PDL coated surfaces and also the neuronalcells on PDL bounded surfaces survived a longer time. On the pattern of covalently bound PDL, neuronalcells and their neurites are confined within the grid line leading to patterned neuronal networks with the long-term survival