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    Formation of an intact liposome layer adsorbed on oxidized gold confirmed by three complementary techniques: QCM-D, AFM and confocal fluorescence microscopy

    Year: 2012

    Journal: Surface and Interface Analysis, Volume 44, Issue 4, pages 426–433, April 2012, 20120618

    Authors: A. P. Serro 1,2, A. Carapeto 1, G. Paiva 1, J. P. S. Farinha 3, R. Colaço 1, B. Saramago 1

    Last authors: B. Saramago

    Organizations: 1 Centro de Química Estrutural, Complexo I, Instituto Superior Técnico, TU Lisbon, Lisboa, Portugal 2 Centro de Investigacão Interdisciplinar Egas Moniz, Instituto Superior de Ciências da Saúde Egas Moniz, Campus Universitário, Quinta da Granja, Monte de Caparica, Caparica, Portugal 3 Centro de Química-Física Molecular and IN-Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico, Lisboa, Portugal

    Country: Portugal

    Supported layers of vesicles of dimyristoyl and dipalmitoyl phosphatidylcholine (DMPC and DPPC) containing cholesterol (CHOL) are adequate models for eukaryotic plasma membranes. Among the possible substrates to support these layers, gold offers the possibility of being used as an electrode for application in sensors. However, the formation of intact liposome layers on gold is not completely understood and several authors use more or less complex strategies to bind the liposomes. In this work we investigate the adsorption of unilamellar vesicles of DMPC, DMPC/CHOL and DMPC/DPPC/CHOL on the surface of oxidized gold using a quartz crystal microbalance with dissipation, atomic force microscopy and laser scanning confocal fluorescence microscopy. The results of all techniques indicate that for lipid concentrations ≥ 0.7 mg⋅mL–1 a dense layer of intact liposomes irreversibly adsorbs on the gold surface.