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    Formation of supported lipid bilayers on indium tin oxide for dynamically-patterned membrane -functionalized microelectrode arrays

    Year: 2009

    Journal: Lab Chip, 2009, 9, 718-725, 20120104

    Authors: Kumar K. 1, Tang CS 1 2, Rossetti FF 1, Textor M 1, Keller B 2,  Vörös J 1, Reimhult E  1*

    Last authors: Erik Reimhult

    Organizations: 1 BioInterface Group, Laboratory of Surface Science and Technology, Swiss Federal Institute of Technology (ETH), Zürich, CH-8093, Zurich, Switzerland. E-mail: erik.reimhult@mat.ethz.ch 2 Swiss Federal Laboratories for Materials Testing and Research (EMPA), CH-8600, Dübendorf, Switzerland

    Country: Switzerland

    Supported lipid bilayers (SLBs) are ideal platforms for the study of membrane proteins and function. Assembly of functional SLBs in an array format would lead to a breakthrough in high-throughput screening of membrane -associated processes, e.g., drugs binding to transmembrane proteins . We report the formation of SLBs from the rupture of anionic vesicles in the presence of Ca2+ ions on ITO-coated surfaces and characterise the assembly and SLB properties. Furthermore, the formation, manipulation and regeneration of SLBs adsorbed on ITO microelectrode array spots using an electric potential switch are demonstrated. This platform enables addressable assembly and the study of electrochemically mediated membrane processes in a microarray format which can be regenerated in situ.