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    Generic Method for Attaching Biomolecules via Avidin–Biotin Complexes Immobilized on Films of Regenerated and Nanofibrillar Cellulose

    Year: 2012

    Journal: Biomacromolecules, 2012, 13 (9), pp 2802–2810, 20120922

    Authors: Hannes Orelma †, Leena-sisko Johansson †, Ilari Filpponen *†, Orlando J. Rojas †‡, and Janne Laine †

    Last authors: Janne Laine

    Organizations: † Department of Forest Products Technology, School of Chemical Technology, Aalto University, Espoo, Finland ‡ Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina 27695, United States

    Country: Finland, USA, US, United States, United States of America, America

    We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated and nanofibrillar cellulose. The dynamics and extent of biomolecular attachment were monitored in situ by quartz crystal microbalance microgravimetry and ex situ via surface analyses with atomic force microscopy and X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) or TEMPO-oxidation significantly increases physisorption of avidins, which can be then covalently conjugated by using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride/N-hydroxysuccinimide (EDS/NHS) coupling chemistries. The developed cellulose–avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA) and antihuman immunoglobulin G (Biotin-anti-hIgG). Finally, we show that cellulose substrates carrying immobilized anti-hIgG are effective in detecting human immunoglobulin G (hIgG) from fluid matrices.