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    How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers

    Year: 2017

    Journal: Adv. Colloid Interface Sci., Volume 243, MAY, page 60–76

    Authors: Boisselier, Elodie; Demers, Eric; Cantin, Line; Salesse, Christian

    Organizations: Natural Sciences and Engineering Research Council of Canada (NSERC); Canadian Institutes for Health Research (CIHR); Canadian National Institute; Reseau de recherche en sante de la vision; Fonds de Recherche du Quebec - Sante (FRQ-S); CIHR

    Keywords: Monolayer; Langmuir films; Protein binding; Retinitis pigmentosa 2; RP2

    This review presents data on the influence of various experimental parameters on the binding of proteins onto Langmuir lipid monolayers. The users of the Langmuir methodology are often unaware of the importance of choosing appropriate experimental conditions to validate the data acquired with this method. The protein Retinitis pigmentosa 2 (RP2) has been used throughout this review to illustrate the influence of these experimental parameters on the data gathered with Langmuir monolayers. The methods detailed in this review include the determination of protein binding parameters from the measurement of adsorption isotherms, infrared spectra of the protein in solution and in monolayers, ellipsometric isotherms and fluorescence micrographs. (C) 2017 Elsevier B.V. All rights reserved.
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