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    Hydrophobin coating prevents Staphylococcus epidermidis biofilm formation on different surfaces

    Year: 2017

    Journal: Biofouling, Volume 33, page 601–611

    Authors: Artini, Marco; Cicatiello, Paola; Ricciardelli, Annarita; Papa, Rosanna; Selan, Laura; Dardano, Principia; Tilotta, Marco; Vrenna, Gianluca; Tutino, Maria Luisa; Giardina, Paola; Parrilli, Ermenegilda

    Organizations: Ministero dell'Universita e della Ricerca Scientifica [PON03PE 00107 1]; Operative National Program Research and Competitiveness [Prot. N. 713/Ric. 29/10/2010]

    Keywords: Protein self-assembly; surface colonization; fungi; biofilm inhibition; confocal laser scanning microscopy; CDC biofilm reactor

    Staphylococcus epidermidis is a significant nosocomial pathogen in predisposed hosts because of its capability of forming a biofilm on indwelling medical devices. The initial stage of biofilm formation has a key role in S. epidermidis abiotic surface colonization. Recently, many strategies have been developed to create new anti-biofilm surfaces able to control bacterial adhesion mechanisms. In this work, the self-assembled amphiphilic layers formed by two fungal hydrophobins (Vmh2 and Pac3) have proven to be able to reduce the biofilm formed by different strains of S. epidermidis on polystyrene surfaces. The reduction in the biofilm thickness on the coated surfaces and the preservation of cell vitality have been demonstrated through confocal laser scanning microscope analysis. Moreover, the anti-biofilm efficiency of the self-assembled layers on different medically relevant materials has also been demonstrated using a CDC biofilm reactor.
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