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    Kinetic behaviour of recombinant Fusarium solani lipases using monomolecular films: Effect of the heterologous expression

    Year: 2017

    Journal: Int. J. Biol. Macromol., Volume 94, JAN, page 319–325

    Authors: Jallouli, Raida; Bouali, Madiha; Gargouri, Youssef; Bezzine, Sofiane

    Organizations: Ministere de l'Enseignement Superieur et de la Recherche

    Keywords: Fusarium solani lipases; Heterologous expression; Monolayer studies; Polyhistidine tag

    Two lipases from Fusarium solani, FSL and FSL2, were efficiently expressed in Pichia pastoris. To check the influence of the expression on interfacial properties of FSL and to study kinetic properties of FSL2, interfacial parameters of FSL2, native FSL, untagged recombinant and tagged recombinant forms of FSL were compared using the monomolecular film technique. Kinetic study on the dependence of the stereoselectivity of these lipases on the surface pressure was performed using three dicaprin isomers spread in the form of monomolecular films at the air-water interface. The FSL2 seems to have an important penetration power with a preference for adjacent ester groups and the heterologous expression accompanied or not with the N-His-tag extension on the FSL were found to modify the pressure preference and increase the catalytic hydrolysis rate of three dicaprin isomers. The heterologous expression was found to preserve the FSL regioselectivity without affecting its stereospecificity at high and low surface pressure. The evaluation of the recombinant expression Effects on Catalysis (REC), the N-Tag Effects on Catalysis (TEC), and the N-Tag and Recombinant expression Effects on Catalysis (TREC) showed that the heterologous expression was more efficient than the presence of the N-terminal tag extension on the FSL. (C) 2016 Elsevier B.V. All rights reserved.
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