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    Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier

    Year: 2011

    Journal: Colloids and Surfaces B: Biointerfaces, 2011, 84 (2), 395-399, 20131009

    Authors: Atsushi Iwanaga, Hitoshi Asakawa, Takeshi Fukuma, Momoka Nakamichi, Sakurako Shigematsu, Markus B. Linder, Tetsuya Haruyama

    Organizations: Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka 808-0196, Japan; Frontier Science Organization, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan; VTT Technical Research Center of Finland, VTT Biotechnology, FIN-02044 VTT, Finland

    A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects.