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    Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains

    Year: 2017

    Journal: Biophys. Chem., Volume 224, MAY, page 20–31

    Authors: Pullmannova, Petra; Pavlikova, Ludmila; Kovacik, Andrej; Sochorova, Michaela; Skolova, Barbora; Slepicka, Petr; Maixner, Jaroslav; Zbytovska, Jarmila; Vavrova, Katerina

    Organizations: European Social Fund; state budget of the Czech Republic [CZ.1.07/2.3.00/30.0061]; Czech Science Foundation [13-23891S]; Charles University in Prague [SVV 260 401]

    The Stratum corneum (SC) prevents water loss from the body and absorption of chemicals. SC intercellular spaces contain ceramides (Cer), free fatty acids (FFA), cholesterol (Chol) and cholesteryl sulfate (CholS). Cer with "very long" acyl chains (for example, N-lignoceroyl-sphingosine, CerNS24) are important for skin barrier function, whereas increased levels of "long" acyl Cer (for example, N-palmitoyl-sphingosine, CerNS16) occur in patients suffering from atopic eczema or psoriasis. We studied the impact of the replacement of CerNS24 by CerNS16 on the barrier properties and microstructure of model SC lipid membranes composed of Cer/FFA/Chol/CholS. Membranes containing the long CerNS16 were significantly more permeable to water (by 38-53%), theophylline (by 50-55%) and indomethacin (by 83-120%) than those containing the very long CerNS24 (either with lignoceric acid or a mixture of long to very long chain FFA). Langmuir monolayers with CerNS24 were more condensed than with CerNS16 and atomic force microscopy showed differences in domain formation. X-ray powder diffraction revealed that CerNS24-based membranes formed one lamellar phase and separated Chol, whereas the CerNS16-based membranes formed up to three phases and Chol. These results suggest that replacement of CerNS24 by CerNS16 has a direct negative impact on membrane structure and permeability. (C) 2017 Elsevier B.V. All rights reserved.
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