Contact
    Start Publications Production of a novel medium chain length ...
    ←  Publication Finder
    Attension

    Production of a novel medium chain length poly(3-hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold

    Year: 2017

    Journal: Microb. Biotechnol., Volume 10, NOV, page 1384–1399

    Authors: Basnett, Pooja; Lukasiewicz, Barbara; Marcello, Elena; Gura, Harpreet K.; Knowles, Jonathan C.; Roy, Ipsita

    Organizations: ReBioStent project - European Union's Seventh Programme for research, technological development and demonstration [604251]; HyMedPoly project-Horizon 2020 [643050]

    This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocinaCH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate) or P(3HHx-3HO-3HD-3HDD). P(3HHx-3HO-3HD-3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES). This study demonstrated that P(3HHx-3HO-3HD-3HDD) was biocompatible with the C2C12 (myoblast) cell line. In fact, the % cell proliferation of C2C12 on the P(3HHx-3HO-3HD-3HDD) scaffold was 72% higher than the standard tissue culture plastic confirming that this novel PHA was indeed a promising new material for soft tissue engineering.
    View publication