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    Response Surface Methodology for Optimizing the Production of Biosurfactant by Candida tropicalis on Industrial Waste Substrates

    Year: 2017

    Journal: Front. Microbiol., Volume 8, FEB 7

    Authors: Almeida, Darne G.; Soares da Silva, Rita de Cassia F.; Luna, Juliana M.; Rufino, Raquel D.; Santos, Valdemir A.; Sarubbo, Leonie A.

    Organizations: State of Pernambuco Foundation for the Assistance to Science and Technology (FACEPE); Research and Development Program of the Brazilian National Electrical Energy Agency (ANEEL); National Council for Scientific and Technological Development (CNPq); Federal Agency for the Support and Evaluation of Graduate Education (CAPES)

    Keywords: biosurfactant; Candida tropicalis; optimization; scale up; oil dispersion

    Biosurfactant production optimization by Candida tropicalis UCP0996 was studied combining central composite rotational design (CCRD) and response surface methodology (RSM). The factors selected for optimization of the culture conditions were sugarcane molasses, corn steep liquor, waste frying oil concentrations and inoculum size. The response variables were surface tension and biosurfactant yield. All factors studied were important within the ranges investigated. The two empirical forecast models developed through RSM were found to be adequate for describing biosurfactant production with regard to surface tension (R-2 = 0.99833) and biosurfactant yield (R-2 = 0.98927) and a very strong, negative, linear correlation was found between the two response variables studied (r = -0.95). The maximum reduction in surface tension and the highest biosurfactant yield were 29.98 mNm(-1) and 4.19 gL(-1), respectively, which were simultaneously obtained under the optimum conditions of 2.5% waste frying oil, 2.5%, corn steep liquor, 2.5% molasses, and 2% inoculum size. To validate the efficiency of the statistically optimized variables, biosurfactant production was also carried out in 2 and 50 L bioreactors, with yields of 5.87 and 7.36 gL(-1), respectively. Finally, the biosurfactant was applied in motor oil dispersion, reaching up to 75% dispersion. Results demonstrated that the CCRD was suitable for identifying the optimumproduction conditions and that the new biosurfactant is a promising dispersant for application in the oil industry.
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