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    Rhamnolipid-aided biodegradation of carbendazim by Rhodococcus sp D-1: Characteristics, products, and phytotoxicity

    Year: 2017

    Journal: Sci. Total Environ., Volume 590, JUL 15, page 343–351

    Authors: Bai, Naling; Wang, Sheng; Abuduaini, Rexiding; Zhang, Meinan; Zhu, Xufen; Zhao, Yuhua

    Organizations: National Natural Science Foundation of China [41271335, 31470191]; Major State Basic Research Development Program of China (973 program) [2015CB150502]

    Keywords: Carbendazim; Rhamnolipid; Biodegradation; Cell surface hydrophobicity; Zeta potential; Detoxification

    We successfully isolated Rhodococcus sp. D-1, an efficient carbendazim-degrading bacterium that degraded 98.20% carbendazim (200 ppm) within 5 days. Carbendazim was first processed into 2-aminobenzimidazole, converted to 2-hydroxybenzimidazole, and then further mineralized by subsequent processing. After genomic analysis, we hypothesized that D-1 may express a new kind of enzyme capable of hydrolyzing carbendazim. In addition, the effect of the biodegradable biosurfactant rhamnolipid on the rate and extent of carbendazim degradation was assessed in batch analyses. Notably, rhamnolipid affected carbendazim biodegradation in a concentration-dependent manner with maximum biodegradation efficiency at 50 ppm (at the critical micelle concentration, CMC) (9733% degradation within 2 days), whereas 150 ppm (3 CMC) rhamnolipid inhibited initial degradation (0.01%, 9926% degradation within 2 and 5 days, respectively). Both carbendazim emulsification and favorable changes in cell surface characteristics likely facilitated its direct uptake and subsequent biodegradation. Moreover, rhamnolipid facilitated carbendazim detoxification. Collectively, these results offer preliminary guidelines for the biological removal of carbendazim from the environment. (C) 2017 Elsevier B.V. All rights reserved.
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