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    Spectroscopic study of fluorescent probes based on G-quadruplex oligonucleotides labeled with ethynylpyrenyldeoxyuridine

    Year: 2017

    Journal: Int. J. Biol. Macromol., Volume 105, DEC, page 862–872

    Authors: Switalska, Angelika; Kierzek, Ryszard; Dembska, Anna; Juskowiak, Bernard

    Organizations: National Science Centre of Poland [2011/01/B/ST4/01188]

    Keywords: G-quadruplex; Ethynylpyrenyldeoxyuridine; Fluorescent probes

    The design, synthesis, and spectral properties of four pyrene labeled oligonucleotide probes with G-quadruplex structure (Tel22-Tpy, Tel22-Upy, Tel22-6Upy, Tel22-18Upy) based on the 22-mer human telomeric sequence (Tel22) have been reported. Pyrene labels in the form of ethynylpyrenyldeoxyuridine have been inserted efficiently into oligodeoxynucleotides probes using phosphoramidite chemistry. The probes exhibited abilities to fold into G-quadruplex structures and to bind metal cations (Na+ and K+). Folding properties of probes and their spectral behavior were examined by recording the UV-vis, fluorescence, and CD spectra as well as by analyzing melting profiles. Fluorescence characteristics and G-quadruplex folding of probes were also studied at the interface of cationic dioctadecyldimethylammonium bromide (DODAB) monolayer. Investigations included film balance measurements (pi-A isotherms) and fluorescence spectra recording using a fiber optic accessory interfaced with a spectrofluorimeter. (C) 2017 Elsevier B.V. All rights reserved.
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