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    Synthesis and application of poly-SNS-anchored carboxylic acid: a novel functional matrix for biomolecule conjugation

    Year: 2011

    Journal: Journal of Materials Chemistry 2011, 21 pp 12337-12343, 20121211

    Authors: Fulya Ekiz , Funda Oguzkaya , Mehriban Akin , Suna Timur , Cihangir Tanyeli and Levent Toppare

    Organizations: Department of Biotechnology, Middle East Technical University, Ankara, Turkey, Department of Chemistry, Middle East Technical University, Ankara, Turkey, Department of Biochemistry, Faculty of Science, Ege University, Izmir, Turkey, Department of Polymer Science and Technology, Middle East Technical University, Ankara, Turkey, TU BITAK Marmara Research Center Energy Institute, Kocaeli, Turkey

    Here we report the synthesis of a novel conducting polymer and its properties as an immobilization platform for biosensor application. The conducting polymer has functional groups used for the formation of amide bonding with the enzyme immobilized on the polymer surface. After covalent immobilization of glucose oxidase (GOx) on the polymeric matrix, its application for glucose biosensing was investigated in detail. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements were used to monitor the surface properties of the polymer before and after biomolecule conjugation. The optimized biosensor showed a very good linearity between 0.01 mM and 1.2 mM, a 13 s response time and a detection limit (LOD) of 0.004 mM to glucose. Also, kinetic parameters, operational and storage stabilities were determined. Apparent Michaelis constant (Kmapp) and Imax values of 1.17 mM and 11.28 µA, respectively, were obtained.