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    The surface modification of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)copolymers to improve the attachment of urothelial cells

    Year: 2013

    Journal: Materials Science and Engineering, C, 33, pp 362-369, 20130926

    Authors: José M. García-García, Isabel Quijada-Garrido, Laura López, Rodrigo París, María Teresa Núñez-López, Enrique de la Peña Zarzuelo, Leoncio Garrido

    Organizations: Departamento de Química-Física de Polímeros, Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva, 3, E-28006, Madrid, Spain, Hospital Universitario Fundación de Alcorcón C/Budapest, 1, E-28922, Alcorcón, Madrid, Spain, Servicio de Urología del Hospital Universitario Fundación de Alcorcón, Avda. Budapest 1, E-28922, Alcorcón, Spain

    Biocompatible and biodegradable poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] substrateswere modified to improve the attachment of porcine urothelial cell culture. The pristine copolymerexhibits excellent mechanical properties to replace the bladder tissue, but its surface lacks chemical functionalitiesto interact with cells. Thus, wet chemical treatments based on NaOH and ethylenediamine in aqueous[ED(aq)] and isopropanol [ED(isoOH)] media to functionalize the P(HB-co-HHx) films surfaces were compared.Among these treatments, short ED(aq) treatment was able to decrease the hydrophobicity, renderinga surface with amino groups and without a significant alteration of the mechanical properties. Furthermore,to enhance the interaction with urothelial cells, laminin derived YIGSR sequence was covalently bound tothese amino functionalized substrates. The focal attachment was clearly improved with this last treatment,comparing with those results found with the unmodified and first-step functionalized P(HB-co-HHx).