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    A Langmuir-Blodgett Study of the Interaction between Amphotericin B and Lipids of Histoplasma capsulatum

    Year: 2022

    Journal: Membranes, Volume 12, MAY

    Authors: Araque-Marin, Pedronel; Naranjo Diaz, Andrea; Gomez Londono, Luisa Fernanda; Jimenez Alzate, Maria del Pilar; Castelli, Francesco; Sarpietro, Maria Grazia; Giordani, Cristiano; Pelaez Jaramillo, Carlos Alberto

    Organizations: Universidad EIA, Envigado, Colombia Convocatoria para el apoyo a proyectos de I+D project [II12017022]; Universidad de Antioquia, Medellin, Colombia Research Center for Exact and Natural Sciences (CIEN in Spanish) [2017-18649]; Administrative Department of Science, Technology and Innovation (Minciencias in Spanish) Bogota, Colombia [111577757781]

    Keywords: histoplasmosis; histoplasma capsulatum; lipid extracts; clinical isolate; environmental isolate; mycelial phase; amphotericin B; langmuir monolayers; molecular organization

    Histoplasma capsulatum is a dimorphic, thermal, and nutritional fungus. In the environment and at an average temperature of 28 degrees C, it develops as a mold that is composed of infecting particles. Once in the host or in cultures at 37 degrees C, it undergoes a transition into the parasitic form. In the present work, we performed chemical extraction and characterization using chromatography techniques of the associated lipid composition of the external surface of the cell wall of the mycelial phase of two isolates of the H. capsulatum: one clinical and one environmental. Several differences were evidenced in the fatty acids in the phospholipid composition. Surface pressure-area isotherms and compression module curves of the Amphotericin B and lipid extract monolayers, as well as (AmB)-lipid extract mixed monolayers were recorded. Results show a high affinity of AmB towards lipid extracts. The most stable monolayers were formed by AmB + environmental with a mass ratio of 1:3 and AmB + clinical with a mass ratio of 1:2. Knowledge of the AmB aggregation processes at a molecular level and the characterization of the lipid extracts allows the possibility to understand the interaction between the AmB and the lipid fractions of H. capsulatum.
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