Contact
    Start Publications An SPR based immunoassay for the sensitive detection of the ...
    ←  Publication Finder
    QSense

    An SPR based immunoassay for the sensitive detection of the soluble epithelial marker E-cadherin

    Year: 2018

    Journal: Nanomed.-Nanotechnol. Biol. Med., Volume 14, OCT, page 1963–1971

    Authors: Vergara, Daniele; Bianco, Monica; Pagano, Rosanna; Priore, Paola; Lunetti, Paola; Guerra, Flora; Bettini, Simona; Carallo, Sonia; Zizzari, Alessandra; Pitotti, Elena; Giotta, Livia; Capobianco, Loredana; Bucci, Cecilia; Valli, Ludovico; Maffia, Michele; Arima, Valentina; Gaballo, Antonio

    Keywords: E-cadherin; Breast cancer; Surface plasmon resonance; Quartz crystal microbalance with dissipation monitoring; Epithelial mesenchymal transition

    Protein biomarkers are important diagnostic tools for cancer and several other diseases. To be validated in a clinical context, a biomarker should satisfy some requirements including the ability to provide reliable information on a pathological state by measuring its expression levels. In parallel, the development of an approach capable of detecting biomarkers with high sensitivity and specificity would be ideally suited for clinical applications. Here, we performed an immune-based label free assay using Surface Plasmon Resonance (SPR)-based detection of the soluble form of E-cadherin, a cell-cell contact protein that is involved in the maintaining of tissue integrity. With this approach, we obtained a specific and quantitative detection of E-cadherin from a few hundred microliters of serum of breast cancer patients by obtaining a 10-fold enhancement in the detection limit over a traditional colorimetric ELISA. (C) 2018 Elsevier Inc. All rights reserved.
    View publication