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    Assessment of 3D-Printed Polycaprolactone, Hydroxyapatite Nanoparticles and Diacrylate Poly(ethylene glycol) Scaffolds for Bone Regeneration

    Year: 2022

    Journal: Pharmaceutics, Volume 14, DEC

    Authors: Sousa, Ana Catarina; Biscaia, Sara; Alvites, Rui; Branquinho, Mariana; Lopes, Bruna; Sousa, Patricia; Valente, Joana; Franco, Margarida; Santos, Jose Domingos; Mendonca, Carla; Atayde, Luis; Alves, Nuno; Mauricio, Ana Colette

    Organizations: Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BD/146172/2019, SFRH/BD/146689/2019, 2021.05265.BD]; Animal Science Studies Centre (CECA), Agroenvironment, Technologies and Sciences Institute (ICETA), Porto University (UP); FCT [PEst-OE/AGR/UI0211/2011]; COMPETE 2020 from ANI-Projetos ID&T Empresas em Copromocao [POCI-01-0247-FEDER-033877, POCI-01-0145-FEDER-031146]; Fundacao para a Ciencia e a Tecnologia (FCT); Centro2020 [UIDB/04044/2020, ROTEIRO/0328/2013]

    Keywords: bone regeneration; critical bone defects; hydroxyapatite nanoparticles; polycaprolactone; diacrylate poly(ethylene glycol); scaffolds

    Notwithstanding the advances achieved in the last decades in the field of synthetic bone substitutes, the development of biodegradable 3D-printed scaffolds with ideal mechanical and biological properties remains an unattained challenge. In the present work, a new approach to produce synthetic bone grafts that mimic complex bone structure is explored. For the first time, three scaffolds of various composition, namely polycaprolactone (PCL), PCL/hydroxyapatite nanoparticles (HANp) and PCL/HANp/diacrylate poly(ethylene glycol) (PEGDA), were manufactured by extrusion. Following the production and characterisation of the scaffolds, an in vitro evaluation was carried out using human dental pulp stem/stromal cells (hDPSCs). Through the findings, it was possible to conclude that, in all groups, the scaffolds were successfully produced presenting networks of interconnected channels, adequate porosity for migration and proliferation of osteoblasts (approximately 50%). Furthermore, according to the in vitro analysis, all groups were considered non-cytotoxic in contact with the cells. Nevertheless, the group with PEGDA revealed hydrophilic properties (15.15 degrees +/- 4.06) and adequate mechanical performance (10.41 MPa +/- 0.934) and demonstrated significantly higher cell viability than the other groups analysed. The scaffolds with PEGDA suggested an increase in cell adhesion and proliferation, thus are more appropriate for bone regeneration. To conclude, findings in this study demonstrated that PCL, HANp and PEGDA scaffolds may have promising effects on bone regeneration and might open new insights for 3D tissue substitutes.
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