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    Binding affinity of family 4 carbohydrate binding module on cellulose films of nanocrystals and nanofibrils

    Year: 2021

    Journal: Carbohydr. Polym., Volume 251, JAN 1

    Authors: Liu, Tian; Zhang, Yu; Lu, Xiaomin; Wang, Peipei; Zhang, Xinyu; Tian, Jing; Wang, Qingcheng; Song, Junlong; Jin, Yongcan; Xiao, Huining

    Organizations: National Natural Science Foundation of China [31730106, 31770623]; Natural Science Foundation of Jiangsu Provincial Universities [17KJA530005]; Priority Academic Program Development of Jiangsu Higher Education Institutions

    Keywords: Cellulose nanocrystal; Cellulose nanofibril; Carbohydrate binding module; Quartz crystal microbalance; Binding mechanism; Thermodynamics

    The binding affinity and thermodynamics of family 4 carbohydrate-binding module (CBM4), belonging to type B CBM, on model surfaces of cellulose nanocrystals (CNC) and nanofibrils (CNF) were investigated by quartz crystal microbalance with dissipation monitoring (QCM-D) technology in real-time at different temperatures. The thermodynamic parameters associated with the interaction, such as Gibbs free energy, enthalpy change, entropy change and heat capacity were obtained using the van't Hoff analysis via a nonlinear parameter estimation. The results demonstrated CBM4 binds preferentially to both CNF and CNC, whereas the driving forces behind them were very different. The former was related to the hydrogen bonds formed in the CBM4 clefts, resulting in a favorable enthalpy but compensated by unfavorable entropy change; on the contrary, the latter was mainly driven by favorable entropy but compensated by unfavorable enthalpic change due to water rearrangement.
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