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    Bio-functionalized titanium surfaces with modified silk fibroin carrying titanium binding motif to enhance the ossific differentiation of MC3T3-E1

    Year: 2021

    Journal: Biotechnol. Bioeng., Volume 118, JUL, page 2585–2596

    Authors: Watanabe, Mai; Bhawal, Ujjal K.; Takemoto, Shinji; Nishiyama, Norihiro; Nakahara, Yuichi; Tatematsu, Ken-Ichiro; Sezutsu, Hideki; Kuwabara, Nobuo; Minamisawa, Tamiko; Shiba, Kiyotaka; Asakura, Tetsuo

    Organizations: Japan Society for the Promotion of Science [23240135, JP19K05609]

    Keywords: 13C solid‐ state NMR; artificial protein; biomaterial; peptide aptamer; transgenic silk fibroin

    Silk fibroin (SF) from Bombyx mori has superior properties as both a textile and a biomaterial, and has been used to functionalize the surfaces of various medical inorganic materials including titanium (Ti). In this study, we endowed SF with reversible binding ability to Ti by embedding a titanium binding motif (minTBP-1 and RKLPDA). Artificial SF proteins were first created by conjugating gene cassettes for SF motif (AGSGAG) and minTBP-1 motif with different ratios, which have been shown to bind reversibly to Ti surfaces in quartz crystal microbalance analyses. Based on these results, the functionalized SF (TiBP-SF) containing the designed peptide [TS[(AGSGAG)(3)AS](2)RKLPDAS](8) was prepared from the cocoon of transgenic B. mori, which accelerates the ossific differentiation of MC3T3-E1 cells when coated on titanium substrates. Thus, TiBP-SF presents an alternative for endowing the surfaces of titanium materials with osseointegration functionality, which would allow the exploration of potential applications in the medical field.
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