Bioactive and adhesive properties of multilayered coatings based on catechol-functionalized chitosan/hyaluronic acid and bioactive glass nanoparticles
Year: 2020
Journal: Int. J. Biol. Macromol., Volume 157, AUG 15, page 119–134
Authors: Almeida, Ana Catarina; Vale, Ana Catarina; Reis, Rui L.; Alves, Natalia M.
Keywords: Natural polysaccharides; Bioactive glass; Multi layered adhesive coatings
Chitosan and hyaluronic add are the most attractive natural polysaccharides used for tissue regeneration, herein innovative orthopedic coatings were constructed by dip-coating technique. Inspired by the tough nacre-like structure, multifunctional (MF) films were constructed using bioactive glass nanopartides (BGNPs) as the inorganic phase and hyaluronic add (HA) and chitosan (CHT) polymers as the organic phase. Polymeric (CTR) films were also built with both polysaccharides. Inspired by the marine mussel's adhesive proteins, it was the first time that multilayered coatings containing both HA and CHT catechol conjugates were combined with BGNPs. Both catechol-conjugates were successful synthesized and, particularly for HA, it was possible to achieve the double of the substitution degree varying the reaction time. Prior to the LbL build-up, viscosity and Zeta potential measurements of the polyelectrolytes were conducted. The in situ LbL growth of the films was monitored by quartz crystal microbalance with dissipation monitoring. It was found that the combination of both catechol conjugates resulted in a more compact LbL structure. It was also shown that MF evidenced bioactivity, CTR presented an improved adhesion, and preliminary cellular tests confirmed the biomedical potential of these multilayered coatings being used in orthopedic implants. (C) 2020 Elsevier B.V. All rights reserved.
