Contact
    Start Publications Cantilever Nanobiosensor Functionalized with Tyrosinase for ...
    ←  Publication Finder
    KSV NIMA

    Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and beta-estradiol in Water

    Year: 2020

    Journal: Appl. Biochem. Biotechnol., Volume 190, APR, page 1512–1524

    Authors: de Cezaro, Alana Marie; Rigo, Aline Andressa; Martinazzo, Janine; Muenchen, Daniela Kunkel; Manzoli, Alexandra; Correa, Daniel Souza; Steffens, Juliana; Steffens, Clarice

    Organizations: Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [00,1]; CnpqConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); FapergsFundacao de Amparo a Ciencia e Tecnologia do Estado do Rio Grande do Sul (FAPERGS); FinepFinanciadora de Inovacao e Pesquisa (Finep)

    Keywords: Water; Contaminants; Hormones; Enzyme; Sensitivity; Limit of detection

    This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17 beta-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/mu g, limit of detection of 0.1 and 0.4 ng/L for the hormones 17 beta-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices.
    View publication