Contact
    Start Publications Chitosan crosslinking with pyridoxal 5-phosphate vitamer toward ...
    ←  Publication Finder
    Attension

    Chitosan crosslinking with pyridoxal 5-phosphate vitamer toward biocompatible hydrogels for in vivo applications

    Year: 2021

    Journal: Int. J. Biol. Macromol., Volume 193, DEC 15, page 1734–1743

    Authors: Craciun, Anda-Mihaela; Mititelu-Tartau, Liliana; Gavril, Gabriela; Marin, Luminita

    Organizations: Ministry of Research, Innovation and Digitization, CNCS/CCCDI - UEFISCDI, within PNCDI III [PD 197/2020]

    Keywords: Hydrogel; Chitosan; Vitamin B6; Controlled biodegradability; Biocompatibility

    Vitamin B6 is an essential micronutrient in the mammalian diet, with role of coenzyme and synergistic effect with some antibiotics and antitumor drugs. Based on these, we hypothesized that its use for the preparation of hydrogels can yield multifunctional biomaterials suitable for in vivo applications. To this aim, chitosan was reacted with the active form of vitamin B6, pyridoxal 5-phosphate, via acid condensation, when clear hydrogels were obtained. Their investigation by structural characterization methods proved that the hydrogelation was a consequence of both covalent imine formation and physical interactions. The novel hydrogels had microporous morphology and showed shrinking effect in phosphate buffer, indicating good shape preservation and slow dissolution in in vivo environment. Their enzymatic biodegradation could be controlled by the imination degree, varying from 40 to 61% in 21 days. They demonstrated very good in vitro cytocompatibility on normal human dermal fibroblasts cells and no harmful effect on experimental mice, confirming their safely use for in vivo application.
    View publication