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    Clinically Relevant Bacterial Outer Membrane Models for Antibiotic Screening Applications

    Year: 2021

    Journal: ACS Infect. Dis., Volume 7, SEP 10, page 2707–2722

    Authors: Mohamed, Zeinab; Shin, Jung-Ho; Ghosh, Surajit; Sharma, Abhishek K.; Pinnock, Ferra; Farnush, Samavi Bint E. Naser; Dorr, Tobias; Daniel, Susan

    Organizations: National Institutes of Health through Venatorx Pharmaceuticals [R01AI136805]; Defense Advanced Research Projects Agency (DARPA) Army Research Office [W911NF-18-2-0152]; National Science Foundation [DGE-1650441, NNCI-2025233]; Howard Hughes Medical Institute [GT11442]; NSF MRSEC program [DMR-1719875]

    Keywords: Gram-negative bacteria; outer membrane vesicle; supported bilayer; membrane interactions; membrane model

    Antibiotic resistance is a growing global health concern that has been increasing in prevalence over the past few decades. In Gram-negative bacteria, the outer membrane is an additional barrier through which antibiotics must traverse to kill the bacterium. In addition, outer membrane features and properties, like membrane surface charge, lipopolysaccharide (LPS) length, and membrane porins, can be altered in response to antibiotics and therefore, further mediate resistance. Model membranes have been used to mimic bacterial membranes to study antibiotic-induced membrane changes but often lack the compositional complexity of the actual outer membrane. Here, we developed a surface-supported membrane platform using outer membrane vesicles (OMVs) from clinically relevant Gram-negative bacteria and use it to characterize membrane biophysical properties and investigate its interaction with antibacterial compounds. We demonstrate that this platform maintains critical features of outer membranes, like fluidity, while retaining complex membrane components, like OMPs and LPS, which are central to membrane-mediated antibiotic resistance. This platform offers a non-pathogenic, cell-free surface to study such phenomena that is compatible with advanced microscopy and surface characterization tools like quartz crystal microbalance. We confirm these OMV bilayers recapitulate membrane interactions (or lack thereof) with the antibiotic compounds polymyxin B, bacitracin, and vancomycin, validating their use as representative models for the bacterial surface. By forming OMV bilayers from different strains, we envision that this platform could be used to investigate underlying biophysical differences in outer membranes leading to resistance, to screen and identify membrane-active antibiotics, or for the development of phage technologies targeting a particular membrane surface component.
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