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    Construction of micro-grooved PCL/nanohydroxyapatite membranes by non-solvent induced phase separation method and its evaluation for use as a substrate for human periodontal ligament fibroblasts

    Year: 2022

    Journal: Chem. Eng. Sci., Volume 248, FEB 2

    Authors: Tuzun-Antepli, Burcu; Elcin, Ayse Eser; Elcin, Yasar Murat

    Keywords: Micro-patterned substrate; Polymer-nanofiller matrix; Periodontal ligament fibroblastic progenitors; Poly(e-caprolactone); Nanohydroxyapatite; Periodontal ligament

    Micro-patterned membrane constructs based on neat, and nanohydroxyapatite (HA)-blended poly(e- caprolactone) were developed using the phase separation micro-molding (PSmM) technique. Physical, chemical, thermal, mechanical and in vitro biological properties of the constructs were evaluated. Human periodontal ligament fibroblasts (hPDLFs) were cultured on the surface of the patterned membranes. The effect of micro-patterning on the morphology and osteogenic differentiation capacity of cells was evaluated by SEM and AR-S staining. SEM findings revealed that hPDLFs were able to adhere to ridges, but generally tended to settle along microgroove pattern channels with multiple filopodia. Micro-groove structure led to a narrower elongation of the cytoplasm, orientation and directionality for migration along the groove. After 30 days of culture, the cells had grown to form multiple layers covering the entire patterned surface, while maintaining the alignment of the cells. Extracellular mineralization and osteogenic differentiation of hPDLFs was demonstrated by high AR-S staining, particularly on HA-blended constructs. (C) 2021 Elsevier Ltd. All rights reserved.
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