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    Correlation between physicochemical characteristics of lignin deposited on autohydrolyzed wood chips and their cellulase enzymatic hydrolysis

    Year: 2022

    Journal: Bioresour. Technol., Volume 350, APR

    Authors: Jia, Wenchao; Shi, Haiqiang; Sheng, Xueru; Guo, Yanzhu; Fatehi, Pedram; Niu, Meihong

    Organizations: Natural Science Foundation of China [31971608]; Natural Science Foundation of Liaoning Province [2019-ZD-0125]; Liaoning Bai-qianwan Talents Program; Innovative Talents in Liaoning Universities and Colleges

    Keywords: Autohydrolysis; Lignin; NMR; Inhibition; Enzyme

    Enzymatic hydrolysis is a method to generate biofuel from biomass, and autohydrolysis is a popular method to pretreat biomass prior to enzymatic hydrolysis. The primary aim of the present study was to determine the role of lignin produced in the autohydrolysis process on the enzymatic hydrolysis of biomass. The HSQC and P-31 NMR analyses confirmed that beta-O-4 of lignin was reduced, while beta-5, beta-beta, and S/G-ratio of lignin were increased with intensifying the hydrolysis intensity. The increase in the hydrolysis intensity significantly enhanced the condensed and non-condensed phenolic OH group of lignin. Interestingly, the cellulase enzyme adsorbed more on lignin that had more phenolic content, and its association with lignin reduced its activity for hydrolyzing cellulose microcrystals. Strong negative correlations were observed between the enzymatic hydrolysis yield and the condensed S-OH (r(2) = 0.978) and G-OH (r(2) = 0.961) of lignin generated in the autohydrolysis process.
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