Contact
    Start Publications Development of a system of heparin multilayers on titanium ...
    ←  Publication Finder
    QSense

    Development of a system of heparin multilayers on titanium surfaces for dual growth factor release

    Year: 2022

    Journal: J. Biomed. Mater. Res. Part A, Volume 110, SEP, page 1599–1615

    Authors: Behrens, Christina; Kauffmann, Philipp; von Hahn, Nikolaus; Giesecke, Ariane; Schirmer, Uwe; Liefeith, Klaus; Schliephake, Henning

    Organizations: German Research Foundation (DFG) [SCHL 168-12/1, LI 916/18-1]

    Keywords: biofunctionalization; bone morphogenic proteins; controlled release; heparin polyelectrolyte multilayer; vascular endothelial growth factor

    The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities. Release characteristics for both growth factors differed significantly depending on film architecture. rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs). rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells. Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs. By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways. In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.
    View publication