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    Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose

    Year: 2017

    Journal: Biomacromolecules, Volume 18, JUN, page 1866–1873

    Authors: Fang, Wenwen; Paananen, Arja; Vitikainen, Marika; Koskela, Salla; Westerholm-Parvinen, Ann; Joensuu, Jussi J.; Landowski, Christopher P.; Penttila, Merja; Linder, Markus B.; Laaksonen, Paivi

    We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.
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