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    Electrostatics affects formation of Watson-Crick complex between DNA bases in monolayers of nucleolipids deposited at a gold electrode surface

    Year: 2021

    Journal: Electrochim. Acta, Volume 390, SEP 10

    Authors: Prieto-Dapena, Francisco; Su, ZhangFei; Alvarez-Malmagro, Julia; Rueda, Manuela; Lipkowski, Jacek

    Organizations: Spain Ministry of Science and Innovation [CTQ2014-57515-C2-1-R, RED2018-102412-T]; Junta de Andalucia [PAI FQM202]; Natural Sciences and Engineering Research Council of Canada (NSERC) [RG03958]

    Keywords: Mixed nucleolipid-phospholipid monolayer; 1,2-Dipalmitoyl-sn-glycero-3-cytidine nucleolipid; Photon modulation infrared reflection absorption spectroscopy (PM-IRRAS); Spectroelectrochemical sensing; Cytosine: guanine comlex

    Chronocoulometry was applied to determine charge in a monolayer of nucleolipid (1,2-dipalmitoyl-sn-glycero-3-(cytidine diphosphate)) deposited at a gold electrode surface. The immersion method was used to measure the potential of zero charge of the interface (E-pzci) which is a sum of charge on the monolayer, of the nucleolipid and charge on the gold surface. Photon polarization infrared reflection absorption spectroscopy (PM-IRRAS) was used to determine formation of the Watson-Crick complex between terminal cytidine moiety of the nucleolipid and guanine (its complementary base) added to the solution. The combination of electrochemical and spectroscopic studies allowed one to demonstrate that the Watson-Crick complex is formed when the interface is positively charged. The potential applied to the electrode affects not only the complex formation but also orientation of the cytosine moiety. The complex is formed when the cytosine moiety is oriented assuming a small angle with respect to the electrode surface. (C) 2021 Elsevier Ltd. All rights reserved.
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