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    Engineering cartilage tissue based on cartilage-derived extracellular matrix cECM/PCL hybrid nanofibrous scaffold

    Year: 2020

    Journal: Mater. Des., Volume 193, AUG

    Authors: Feng, Bei; Ji, Tianji; Wang, Xingang; Fu, Wei; Ye, Lincai; Zhang, Hao; Li, Fen

    Organizations: National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81770332, 81601622]; Fund of Transformation Medicine Cross Research of Shanghai Jiao Tong University [YG2019ZDA04]; Program for Outstanding Medical Academic Leader [2019LJ22]

    Keywords: Extracellular matrix (ECM); Polycaprolactone (PCL); Electrospinning; Hybrid nanofibers; Cartilage regeneration

    Extracellular matrix (ECM) derived from decellularized tissues and organs has been used as a biological scaffold in a variety of pre-clinical and clinical applications. However, the lack of mechanical properties and shape controllability is a drawback. In contrast, synthetic polymers can be easily engineered with good mechanical performance, but they have limited biological functionality. In this study, we explored a new method to electrospun cartilage-derived extracellular matrix (cECM) and polycaprolactone (PCL) composite nanofibrous membranes. The cartilage is a compact tissue for which electrospinning is difficult. To overcome this problem, the cartilage was sliced into pieces, milled into powders, and digested into a looser structure. The cECM/PCL (mass ratio 50:50) hybrid nanofibers appeared to be smooth, thinner, and uniform with enhanced mechanical properties and wettability compared to the electrospun PCL. Meanwhile, the presence of cECM in the cECM/PCL nanofibrous membranes significantly promoted chondrocyte proliferation in vitro and facilitated cartilage regeneration in vivo. All these results indicate that the cECM/PCL nanofibrous membranes fabricated with good mechanical properties and biocompatibility may represent a promising scaffold for cartilage regeneration. Moreover, this work may offer a convenient and cost-effective method to synthesize ECM-based hybrid nanofibrous scaffolds for other tissue applications. (c) 2020 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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