Contact
    Start Publications Engineering Stem Cell Recruitment and Osteoinduction via ...
    ←  Publication Finder
    Attension

    Engineering Stem Cell Recruitment and Osteoinduction via Bioadhesive Molecular Mimics to Improve Osteoporotic Bone-Implant Integration

    Year: 2022

    Journal: Research, Volume 2022, SEP 6

    Authors: Bai, Jiaxiang; Ge, Gaoran; Wang, Qing; Li, Wenming; Zheng, Kai; Xu, Yaozeng; Yang, Huilin; Pan, Guoqing; Geng, Dechun

    Organizations: National Natural Science Foundation of China [32222041, 82072425, 82072498, 21875092]; Natural Science Foundation of Jiangsu Province [BK20220059, BE2020666]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [LCZX202003]; Special Project of Diag-nosis and Treatment Technology for Key Clinical Diseases in Suzhou [LCZX202003]; Jiangsu Specially Appointed Professor Program; Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX22_3217]

    For patients with osteoporosis, the therapeutic outcomes of osteoimplants are substantially affected by the impaired proliferation, migration, and osteogenic differentiation abilities of bone marrow mesenchymal stem cells (BMSCs). To improve bone-implant integration in osteoporotic condition, here we reported a one-step biomimetic surface strategy to introduce BMSC recruiting and osteoinductive abilities onto metallic osteoimplants. In our design, the bioadhesive molecular peptide mimic inspired by mussel foot proteins (Mfps) was used as molecular bridging for surface functionalization. Specifically, a BMSC-targeting peptide sequence (E7) and an osteogenic growth peptide (Y5) were grafted onto the titanium implant surfaces through a mussel adhesion mechanism. We found that a rational E7/Y5 feeding ratio could lead to an optimal dual functionalization capable of not only significantly improving the biocompatibility of the implant but also enabling it to recruit endogenous BMSCs for colonization, proliferation, and osteogenic differentiation. Mechanistically, the E7-assisted in situ recruitment of endogenous BMSCs as well as the enhanced interfacial osteogenesis and osteointegration was associated with activation of the C-X-C chemokine receptor type 4 (CXCR4) receptor on the cell surface and promotion of stromal cell-derived factor (SDF-1 alpha) autocrine secretion. We anticipated that rational dual-functional surfaces through bioadhesive molecular mimics will provide a simple, effective, nonimmunogenic, and safe means to improve the clinical outcomes of intraosseous implants, especially under osteoporotic conditions.
    View publication