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    Enrichment of IgG and HRP glycoprotein by dipeptide-based polymeric material

    Year: 2022

    Journal: Talanta, Volume 241, MAY 1

    Authors: Zheng, Xintong; Zhang, Fusheng; Zhao, Yanyan; Xiong, Yuting; Zhang, Xiaoyu; Shi, Zhenqiang; Qian, Shengxu; Qin, Haijuan; Qing, Guangyan

    Organizations: National Natural Science Foundation of China [21775116, 21922411]; DICP Innovation Funding [DICPRC201801, I202008]; LiaoNing Revitalization Talents Program [XLYC1802109]

    Separation, purification, and identification of glycoproteins are essential for understanding their vital roles in biological and pathological processes. However, glycoproteins are difficult to be captured due to their low abundance, strong interference from non-glycosylated proteins. Here, we report a promising dipeptidebased saccharide recognition platform to selectively enrich two typical glycoproteins, named immunoglobin G (IgG) and horseradish peroxidase (HRP). Different from the conventional glycoprotein enrichment method based on boronic acid affinity or hydrophilic interaction with glycans, the present method was established based on affinity between Pro-Glu (PE) dipeptide and mannose, which is a key unit in the pentasaccharide core of the IgG and HRP glycans. The prepared PE homopolymer surface was proved to selectively bind IgG and HRP superior to that of bovine serum albumin (BSA). Benefiting from this feature, selective enrichment of IgG and HRP was achieved from a protein mixture containing 200-fold BSA interference by using polyPE@SiO2 under a dispersive solid-phase extraction (dSPE) mode. High adsorption capacity, controllable and selective adsorption behaviors, as well as satisfactory recovery demonstrated the high potential of the dipeptidebased polymeric material in IgG and HRP enrichment. This study might provide a new insight to solve the challenging problem of glycoprotein separation.
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