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    Evaluation of Human Osteoblasts on NIPS Micro-Patterned PCL Carriers Containing Nanohydroxyapatite and Reduced Graphene Oxide Using PS mu M

    Year: 2022

    Journal: Molecules, Volume 27, OCT

    Authors: Tuzun-Antepli, Burcu; Seker, Sukran; Elcin, Ayse Eser; Khang, Gilson; Elcin, Yasar Murat

    Organizations: Scientific and Technological Research Council of Turkiye (TUBITAK) [119N136]

    Keywords: phase separation micromolding; micropatterned cell carrier; osteoblasts; poly(epsilon-caprolactone); nanohydroxyapatite; reduced graphene oxide; osteogenic differentiation

    The content and surface topology of tissue engineering scaffolds are two important parameters in regulating the cell behavior. In this study, a phase separation micromolding (PS mu M) method was implemented to develop micro-groove-imprinted poly(E-caprolactone) (PCL)-nano hydroxyapatite (nHAp)-reduced graphene oxide (rGO) ternary blend constructs. Physical and chemical characterizations of cell-devoid constructs were performed by FTIR, XRD, TGA, DSC, porosity, swelling, wettability analysis, tensile and compression mechanical tests. The in vitro biological performance of human osteoblasts cultured on micro-patterned blend constructs was evaluated by MTT and alamarBlue viability assays. The findings revealed that nHAp and rGO significantly promote cell viability and proliferation, while the micro-pattern determines the direction of cell migration. Alkaline phosphatase and Ca2+ analyses were carried out to determine the osteogenic properties of cell-laden constructs. This study describes a simple method to generate topologically modified ternary blend PCL/nHAp/rGO constructs using the PS mu M method, which contributes to cell proliferation and migration, which is particularly important in regenerative medicine.
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