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    High Axial and Lateral Resolutions on Self-Assembled Gold Nanoparticle Metasurfaces for Live-Cell Imaging

    Year: 2020

    Journal: ACS Appl. Nano Mater., Volume 3, NOV 25, page 11135–11142

    Authors: Masuda, S; Kuboki, T; Kidoaki, S; Lee, ST; Ryuzaki, S; Okamoto, K; Arima, Y; Tamada, K

    Organizations: JSPS KAKENHI in JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [19H05627]

    Keywords: plasmonic metasurface; gold nanoparticles; self-assembly; live-cell imaging; high resolution

    A plasmonic metasurface composed of homogeneously self-assembled gold nanoparticles can provide high-contrast fluorescence images confined to the nanointerface. In this study, we successfully demonstrated real-time, high-spatiotemporal-resolution imaging of adhered Venus-paxillin-3T3 live cells under a widefield microscope, where not only a high axial resolution but also a high lateral resolution down to the theoretical limit were confirmed through nascent cluster formation of paxillin. The improved lateral resolution on the sheet could be interpreted as the characteristic of localized surface plasmon resonance (LSPR)-mediated enhanced fluorescence and the metasurface acting as a nanothickness plane light emitter. We also found minimized photobleaching, owing to the increase in the emission efficiency via plasmon-exciton coupling. This simple nanomaterial-based technique will be a powerful tool to enhance interfacial signals and improve the quality of live-cell images, not only under widefield microscopes but also in combination with various super-resolution microscope systems in the future.
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