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    High-resolution label-free studies of molecular distribution and orientation in ultrathin, multicomponent model membranes with infrared nano-spectroscopy AFM-IR

    Year: 2019

    Journal: J. Colloid Interface Sci., Volume 542, 1-Apr, page 347–354

    Authors: Lipiec, Ewelina; Wnetrzak, Anita; Chachaj-Brekiesz, Anna; Kwiatek, Wojciech; Dynarowicz-Latka, Patrycja

    Organizations: Malopolska Regional Operational Programme Measure 5.1 Krakow Metropolitan Area as an important hub of the European Research Area [MRPO.05.01.00-12-013/15]; National Science Center of the Republic of Poland [2014/13/D/NZ1/01014]

    Keywords: Cyclosporin A; Lipid rafts; Langmuir-Blodgett films; AFM-IR; Phase separation

    Biological membranes are undoubtedly very interesting systems. Unfortunately, their analysis is very complicated and therefore simplified artificial models, like Langmuir monolayers, are frequently applied. In this work a novel method of label-free monomolecular films analysis based on infrared nano-spectroscopy (AFM-IR) is presented. In order to verify applicability of this approach well-defined referential system of sphingomyelin (SM), cholesterol (Chol) and cyclosporin A (CsA) is applied. AFM-IR method allows to directly and chemoselectively map the distribution of components in model lipid membranes. Additionally the orientation of Chol and SM molecules in the monolayer is determined by application of two perpendicular infrared laser polarizations. This paper is the first report of using AFM-IR to analyze LB films with extremely high sensitivity. (C) 2019 Published by Elsevier Inc.
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