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    High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder

    Year: 2017

    Journal: Biosens. Bioelectron., Volume 87, JAN 15, page 164–170

    Authors: Hwang, Hye Jin; Ryu, Myung Yi; Park, Chan Young; Ahn, Junki; Park, Hyun Gyu; Choi, Changsun; Ha, Sang-Do; Park, Tae Jung; Park, Jong Pil

    Keywords: Human norovirus; Affinity peptide; Molecular binder; Limit of detection; Sensitivity; Biosensor

    Norovirus is known as the major cause of highly infection for gastrointestinal tracts. In this study, robust and highly sensitive biosensors for detecting human norovirus by employing a recognition affinity peptide-based electrochemical platform were described. A series of amino acid-substituted and cysteine-incorporated recognition peptides isolated from evolutionary phage display technique was chemically synthesized and immobilized to a gold sensor layer, the detection performance of the gold-immobilized synthetic peptide-based sensor system was assessed using QCM, CV and EIS. Using EIS, the limit of detection with Noro-1 as a molecular binder was found to be 99.8 nM for recombinant noroviral capsid proteins (rP2) and 7.8 copies/mL for human norovirus, thereby demonstrating a high degree of sensitivity for their corresponding targets. These results suggest that a biosensor which consists of affinity peptides as a molecular binder and miniaturized microdevices as diagnostic tool could be served as a new type of biosensing platform for point-of-care testing. (C) 2016 Elsevier B.V. All rights reserved.
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