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    Improving the Viability of Probiotics under Harsh Conditions by the Formation of Biofilm on Electrospun Nanofiber Mat

    Year: 2022

    Journal: Foods, Volume 11, MAY

    Authors: Shi, Jiao; Li, Shu-Fang; Feng, Kun; Han, Shuang-Yan; Hu, Teng-Gen; Wu, Hong

    Organizations: Science and Technology Project of Guangzhou City [202206010165]; Guangdong Major Project of Basic and Applied Basic Research [2020B0301030005]; Natural Science Foundation of Guangdong Province [2021A1515010765]; Key-Area Research and Development Program of Guangdong Province [2020B020226007]; Collaborative Innovation Center for Sports of Guangdong Province [2019B110210004]

    Keywords: electrospun nanofiber mat; biofilm; probiotics; viability; luxS gene

    For improving probiotics' survivability under harsh conditions, this study used Lactiplantibacillus plantarum GIM1.648 as a model microorganism to investigate its ability to produce biofilms on electrospun ethyl cellulose nanofiber mats. SEM observations confirmed that biofilm was successfully formed on the nanofibers, with the latter being an excellent scaffold material. The optimal cultivation conditions for biofilm formation were MRS medium without Tween 80, a culture time of 36 h, a temperature of 30 degrees C, a pH of 6.5, and an inoculum concentration of 1% (v/v). The sessile cells in the biofilm exhibited improved gastrointestinal and thermal tolerance compared to the planktonic cells. Additionally, the RT-qPCR assay indicated that the luxS gene played a crucial role in biofilm formation, with its relative expression level being 8.7-fold higher compared to the planktonic cells. In conclusion, biofilm formation on electrospun nanofiber mat has great potential for improving the viability of probiotic cells under harsh conditions.
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