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    In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold

    Year: 2022

    Journal: J. Adv. Res., Volume 35, JAN, page 13–24

    Authors: Shuai, Cijun; Peng, Bo; Feng, Pei; Yu, Li; Lai, Ruilin; Min, Anjie

    Organizations: Natural Science Foundation of China [51905553, 51935014, 82072084, 81871498]; Hunan Provincial Natural Science Foundation of China [2019JJ50774, 2018JJ3671, 2019JJ50588, 2017JJ2392]; Provincial Key R & D Projects of Jiangxi [20201BBE51012]; JiangXi Provincial Natural Science Foundation of China [20192ACB20005]; Project of Hunan Provincial Science and Technology Plan [2017RS3008]; Project of State Key Laboratory of High Performance Complex Manufacturing, Central South University; Shenzhen Science and Technology Plan Project [JCYJ20170817112445033]; Technology Innovation Plat-form Project of Shenzhen institute of Information Technology 2020 [PT2020E002]; Scientific research project of Hunan provincial health commission [B20180054]; Changsha science and technology project [kq1706072]

    Keywords: In situ synthesis; GO; HAP; Mechanical properties; Bioactivity

    Introduction: It is urgently needed to develop composite bone scaffold with excellent mechanical properties and bioactivity in bone tissue engineering. Combining graphene oxide (GO) and hydroxyapatite (HAP) for the reinforcement of biopolymer bone scaffold has emerged as a promising strategy. However, the dispersion of GO and HAP remains to be a big challenge. Objectives: In this present work, the mechanical properties of GO and the bioactivity of and HAP were combined respectively via in situ synthesis for reinforcing biopolymer bone scaffold. Methods: GO nanosheets were employed to in situ synthesize GO-HAP nanocomposite via hydrothermal reaction, in which their abundant oxygen-containing groups served as anchor sites for the chelation of Ca2+ and then Ca2+ absorbed HPO42- via electrovalent bonding to form homogeneously dispersed HAP nanorods. Thereby, the GO-HAP nanocomposite was blended with biopolymer poly-L-lactic acid (PLLA) for fabricating biopolymer scaffold by selective laser sintering (SLS). Results: GO nanosheets were uniformly decorated with HAP nanorods, which were about 60 nm in length and 5 nm in diameter. The compressive strength and modulus of PLLA/12% GO-HAP were significantly increased by 53.71% and 98.80% compared to the pure PLLA scaffold, respectively, explained on the base of pull out, crack bridging, deflection and pinning mechanisms. Meanwhile, the mineralization experiments indicated the PLLA/GO-HAP scaffold displayed good bioactivity by inducing the formation of apatite layer. Besides, cell culturing experiments demonstrated the favorable cytocompatibility of scaffold by promoting cell adhesion and proliferation. Conclusions: The present findings show the potential of PLLA/GO-HAP composite scaffold via in situ synthesis in bone tissue engineering. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of Cairo University.
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