Contact
    Start Publications Interaction of chlorogenic acid with model lipid membranes and ...
    ←  Publication Finder
    KSV NIMA

    Interaction of chlorogenic acid with model lipid membranes and its influence on antiradical activity

    Year: 2021

    Journal: Biochim. Biophys. Acta-Biomembr., Volume 1863, JAN 1

    Authors: Cejas, J. P.; Rosa, A. S.; Nazareno, M. A.; Disalvo, E. A.; Frias, M. A.

    Organizations: ANPCyT [PICT 2015-1111]; CONICET (RA)

    Keywords: Chlorogenic acid, lipid monolayers; Antiradical activity; Surface pressure; FTIR-ATR

    Chlorogenic acid (CGA) is a strong phenolic antioxidant with antibacterial properties composed by a caffeoyl ester of quinic acid. Although a number of benefits has been reported and related to interactions with the red blood cell membranes, details on its membrane action and how composition and membrane state may affect it, is not yet well defined. In this work, the interaction of CGA with lipid monolayers and bilayers composed by 1,2-dimiristoyl-snglycero-3-phosphocholine (DMPC); 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine (14:0 diether PC); 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine (16:0 diether PC) were studied at different surface pressures (pi). The kinetics of interaction was found to be more rapid in DMPC than in the absence of carbonyl groups. Measurements by FTIR-ATR at different water activities confirm specific interactions of CGA with carbonyl and phosphate groups affecting water level along hydrocarbon region. The antioxidant activity of CGA in the presence of DMPC unilamellar vesicles, evidenced by the absorbance reduction of the radical cation ABTS(center dot+), is significantly different with respect to aqueous solution. The influence of CGA on antiradical activity (ARA) with lipid membranes depending on the hydration state of the lipid interface is discussed.
    View publication